Part:BBa_K4139024

CAP-FP

This construct starts with enhanced green fluorescent protein (eGFP) followed by our chimeric anti-PbEL04 (CAPE) which is a complementary to PbEL04. After our CAPE region is our chimeric anti Pro1 (CAP) region which is complementary to the Pro1 protein. Finally, after the CAP region is a His-tag to help with purification. This constructs’ function is to be a bi-spicific protein that can bind to both PbEL04 and Pro1. We first made this coding region as a basic part (BBa_K4139017) which we have made as a composite part with a ribosome binding site, promoter, and terminator.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1767
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 27
  Illegal NheI site found at 50
  Illegal PstI site found at 1767
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1949
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1767
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1767
  Illegal AgeI site found at 529
  
• 1000
  COMPATIBLE WITH RFC[1000]